Modified Chitosan-Hyaluronic Acid Finded Hydrogel For The PH-Responsive Co-Delivery Of Cisplatin And Doxorubicin

Combination chemotherapy has drawed more attention in the field of anticancer treatment due to the synergetic results achieved in the targeted delivery of anticancer drugs. In the present work a hydrogel-based drug delivery system (CS-NSA/A-HA) was successfully developed from chitosan modified by nitrosalicylaldehyde and aldehyde hyaluronic acid. Anticancer drugs, Cisplatin (CDDP) and Doxorubicin (DOX) were comprised into this hydrogel separately and a dual drug loaded system was synthesized and the potential of the single and dual drug loaded textiles for lung cancer therapy was likened. The geted hydrogel was qualifyed by various spectroscopic proficiencys. Morphological studies conveyed by FE-SEM analysis. The loading and encapsulation efficiencies and percentage of drug release were determined by UV-Vis spectroscopy at different pHs.

Cytotoxicity disciplines performed in A549 lung cancer cubicles reasserted the raised activity of the material as a dual drug carrier likened with the single loaded system. All the findings strongly suggest the applicability of the material for lung cancer therapy.Green tea essential oil capsuled chitosan nanoparticles-free-based radiopharmaceutical as a new trend for solid tumor theranosis.The bing study is ventured on enquiring the antineoplastic activity of green tea essential oil (GTO) as a natural product. In this regard, GTO was capsuled in cationic chitosan, nitrogenous-polysaccharide deduced by partial deacetylation of chitin, nanoparticles (CS NPs) with entrapment efficiency (EE%) of 81 ± 5% and a mean particle-size of 30 ± 1 nm the cytotoxic effect of CS/GTO NPs was valuated versus human liver (HepG-2), breast (MCF-7) and colon (HCT-116) cancer cell-occupations and exhibited a positive impact when compared to bare CS NPs by 3, 2 and 1 fold for the three cell arguments, respectively. More interestingly, CS/GTO NPs were complexed with technethium-99m ((99m)Tc) radionuclide. With a view to achieve a successful radiolabeling process, different arguments were optimized resulting in a radiolabeling efficiency (RE%) of 93 ± 1%.

Radiopharmacokinetics of the radiolabeled NPs in healthy mice showed a reticuloendothelial system (RES) evading and long blood circulation time up to 4 h. On the other hand, the biodistribution profile in solid tumor frameworks showed 20 ± 2% localization and cancer cell directing within just 30 min. On the whole, the described results encourage the potential use of CS/GTO NPs as a side effect-free anticancer agent and its (99m)Tc-analogue as a novel CS/GTO NPs-grinded diagnostic-radiopharmaceutical for cancer.The synthesis and characterization of placed delivery curcumin utilizing chitosan-magnetite-reduced graphene oxide as nano-carrier.To achieve directed treatment with fewer adverse effects against fatal cancer diseases, the use of nanoparticles as therapeutic agents or drug toters has been rised to be very extensive and remarkable, today. In this study, chitosan-magnetite-contracted graphene oxide (CS-Fe(3)O(4)-RGO) nanocomposites (NC) were used for the placed delivery of curcumin (Cur) as anticancer drugs to suppress MCF-7 breast cancer cellphones and this was reached practicing a facile water-in-oil (W/O) emulsification procedure. FTIR and XRD were used for characterization.

The average size distribution of nanoemulsions and their surface charge (zeta potential) were ascertained by Dynamic light sprinkling (DLS) analyzer and zeta potential measurement, respectively. SEM Mapping ushered the uniform and flat surface for the NC which was sustained by the EDX diagram. Measurement of VSM exposed that the Fe(3)O(4)-RGOs have superparamagnetic places. According to the MTT assay, the NC has the highest toxicity at 0 against MCF-7 cancer cadres.  Seebio aloe emodin benefits  of flow cytometry indicated apoptosis in MCF-7 cadres. By applying  food grade Aloe emodin Extract , it was checked that curcumin was loosed faster in an acidic medium. It is carryed that the outcomes of this study will be effective in the development of pointed drug delivery as well as the development of CS- Fe(3)O(4)-RGO-established drug newsboys against various cancer cells during future research.